| Autor: |
Bannikova GE, Stolbushkina PP, Drozd NN, Vikhoreva GA, Varlamov VP, Makarov VA, Panov AV |
| Jazyk: |
ruština |
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia [Prikl Biokhim Mikrobiol] 2004 Jul-Aug; Vol. 40 (4), pp. 429-34. |
| Abstrakt: |
The possibility of obtaining low-molecular-weight heparins using the chitinolytic enzymatic complex immobilized on Silochrom has been demonstrated. The optimal conditions of this process (sodium acetate buffer, pH 7.0-7.5; temperature, 40-45 degrees C; and duration of hydrolysis, 3 h) were determined. Depending on the ratio between heparin and the immobilized enzymatic complex, samples with molecular weight varying from 1.7 to 4.7 kDa, were obtained. These complexes inhibited factor Xa in 2.0-3.7 times more effectively than original heparin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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