Parallel single molecule detection with a fully integrated single-photon 2x2 CMOS detector array.
| Autor: | Gösch M; Karolinska Institute, Department of Medical Biochemistry and Biophysics, S-171 77 Stockholm, Sweden., Serov A, Anhut T, Lasser T, Rochas A, Besse PA, Popovic RS, Blom H, Rigler R |
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| Jazyk: | angličtina |
| Zdroj: | Journal of biomedical optics [J Biomed Opt] 2004 Sep-Oct; Vol. 9 (5), pp. 913-21. |
| DOI: | 10.1117/1.1781668 |
| Abstrakt: | We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2x2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed. ((c) 2004 Society of Photo-Optical Instrumentation Engineers.) |
| Databáze: | MEDLINE |
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