| Autor: |
Foster DN; Department of Poultry Science, Ohio State University, Wooster 44691., Galehouse D, Giordano T, Min B, Lamb IC, Porter DA, Intehar KJ, Bacon WL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of molecular endocrinology [J Mol Endocrinol] 1992 Feb; Vol. 8 (1), pp. 21-7. |
| DOI: |
10.1677/jme.0.0080021 |
| Abstrakt: |
Recombinant cDNA clones that encode the alpha subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754 bp in length. It contained 81 nucleotides of the 5'-untranslated region (UTR), an open-reading frame of 360 bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature alpha subunit, and 268 nucleotides of the 3'-UTR, followed by a 45 bp poly(A) tract. There was 69-79% homology between the nucleotide sequence of the coding region for the chicken and mammalian alpha-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp alpha-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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