| Autor: |
Fischbach C; Department of Pharmaceutical Technology, University of Regensburg, 93040 Regensburg, Germany., Spruss T, Weiser B, Neubauer M, Becker C, Hacker M, Göpferich A, Blunk T |
| Jazyk: |
angličtina |
| Zdroj: |
Experimental cell research [Exp Cell Res] 2004 Oct 15; Vol. 300 (1), pp. 54-64. |
| DOI: |
10.1016/j.yexcr.2004.05.036 |
| Abstrakt: |
Tissue-inherent factors such as cell-cell and cell-extracellular matrix interactions are regarded to exert a potentially large impact on adipogenesis as well as on secretory functions of adipose tissue. However, an appropriate 3-D adipogenesis model useful for addressing such interactions is still lacking. In this study, using tissue-engineering techniques, we demonstrate for the first time the development of coherent fat pads consisting of unilocular signet-ring cells in vitro. The constructs were generated by differentiating 3T3-L1 preadipocytes on 3-D polymeric scaffolds for either 9, 21, or 35 days in vitro. Only long-term culture yielded uniform tissues histologically comparable to native fat. Light and scanning electron microscopy provided direct evidence of 3-D tissue coherence and cell-cell contact in a tissue context, which was in strong contrast to conventional 2-D monolayer culture. Further differences between the two culture systems included enhanced secretion of leptin in 3-D tissue culture and differences in laminin expression (mRNA and protein level). Increase of triglyceride content over culture time and mRNA expression of other adipocyte genes, such as PPARgamma and Glut-4, were found to be similar. Implantation of long-term differentiated tissue constructs in nude mice resulted in further development and maintenance of fat pads. The presented model system is suggested to contribute to a better understanding of adipose tissue development and function facilitating studies on tissue-inherent interactions in vitro and in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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