| Autor: |
Iyer YS; Institute of Immunohaematology, 13th floor, New Multistorey-Building, KEM hospital compound, Parel, Mumbai-400 012, India., Vasantha K, Joshi SR, Patwardhan M, Pujari V, Jadhav S, Mohanty D |
| Jazyk: |
angličtina |
| Zdroj: |
Immunohematology [Immunohematology] 2004; Vol. 20 (1), pp. 59-62. |
| Abstrakt: |
While studying the serologic characteristics of certain monoclonal blood group antibodies, we observed a hybridoma clone (5A-11E10) with anti-N-like serologic specificity that was dependent on the presence of the bicarbonate anion. The diluted cell culture supernatant preferentially agglutinated M-N+ RBCs by immediate spin. This supernatant also agglutinated M-N+ RBCs that had been treated with trypsin or sialidase (to remove N-reactivity), suggesting anti-'N' activity. Anti-'N' specificity was confirmed by the supernatant's non-reactivity with N+ RBCs treated with papain (to remove 'N' reactivity) or with ('N'-negative) M+N-U- RBCs. The requirement for bicarbonate in the MoAb's formulation was not a function of pH. Both sodium and ammonium bicarbonate supported agglutination, but neither sulfate nor carbonate was effective. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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