| Autor: |
Arêas AP; Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil., Oliveira ML, Miyaji EN, Leite LC, Aires KA, Dias WO, Ho PL |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2004 Aug 13; Vol. 321 (1), pp. 192-6. |
| DOI: |
10.1016/j.bbrc.2004.06.118 |
| Abstrakt: |
Cholera toxin B subunit (CTB) is responsible for CT holotoxin binding to the cell and has been described as a mucosal adjuvant for vaccines. In this work, the ctxB gene was genetically fused to the psaA gene from Streptococcus pneumoniae, a surface protein involved in its colonization in the host that is also considered a vaccine antigen candidate against this pathogen. The CTB-PsaA fusion protein was expressed in Escherichia coli, and the purified protein was used for intranasal immunization experiments in Balb/C mice. CTB-PsaA was able to induce both systemic and mucosal antibodies evaluated in serum, saliva, and in nasal and bronchial wash samples, showing that CTB-PsaA is a promising molecule to be investigated as S. pneumoniae vaccine antigen candidate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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