| Autor: |
Farnia P; Iranian National reference TB Laboratory, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shaheed Beheshti University of Medical Sciences and Health Services, Darabad, Tehran 19556, Iran. pfarnia@hotmail.com, Mohammadi F, Mirsaedi M, Zia Zarifi A, Tabatabee J, Bahadori M, Akbar Velayati A, Reza Masjedi M |
| Jazyk: |
angličtina |
| Zdroj: |
Microbes and infection [Microbes Infect] 2004 Sep; Vol. 6 (11), pp. 972-6. |
| DOI: |
10.1016/j.micinf.2004.04.017 |
| Abstrakt: |
The viability of Mycobacterium tuberculosis (MTB) in serial sputum specimens from persistently smear positive patients was evaluated. The assay was based on oxidation-reduction of Alamar Blue and Malachite Green dyes that change their color in response to MTB growth. A total of 280 sputum specimens from 40 persistently smear positive TB patients and 40 sputa from non-tuberculosis patients were digested, decontaminated and examined microscopically. To check the MTB viability, the sediments from decontaminated samples were inoculated into three culture media: Lowenstein-Jensen (LJ) slants, Alamar Blue and Malachite Green culture tubes. We found that out of 280 smear positive specimens, the LJ culture was positive in 124 (44%). The numbers of correctly identified S+/C+ cases by Alamar Blue and Malachite Green were 118 (95%) and 116 (93%), respectively. The mean time required for reporting the positive signal in Alamar Blue culture tubes was 9 versus 11 days by Malachite Green culture tubes. In the standard LJ culture media the average detection time was 27 days (P < 0.05). The sensitivity of LJ was 99%, Alamar Blue 95% and Malachite Green 93%. The specificity was 100%, 92% and 93%, respectively. The oxidation-reduction method is rapid, sensitive and inexpensive in monitoring the treatment response of patients with pulmonary TB. Thus, using this method can be of paramount importance, particularly in resource-constrained areas. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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