| Autor: |
Case ME; Department of Genetics, University of Georgia, Athens 30602., Geever RF, Asch DK |
| Jazyk: |
angličtina |
| Zdroj: |
Genetics [Genetics] 1992 Apr; Vol. 130 (4), pp. 729-36. |
| DOI: |
10.1093/genetics/130.4.729 |
| Abstrakt: |
Gene replacement by transformation, employing selective genetic recombination techniques, has been used to delete or disrupt the qa-x, qa-y and qa-1S genes of the qa gene cluster of Neurospora crassa. The growth characteristics of the strain carrying the deletion of the qa-y gene support earlier evidence that this gene encodes a quinic acid permease. The strain containing the deletion of the qa-1S gene (delta qa-1S) was examined with respect to quinic acid induction and carbon catabolite repression. The delta qa-1S strain exhibits constitutive expression of the qa genes supporting earlier evidence that the qa-1S gene codes for a repressor. Several of the qa genes continued to be expressed at high levels even in the presence of glucose in the delta qa-1S strain, which indicates that transcription of these genes is not being affected directly by a repressor molecule in the presence of glucose. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
