[Expression of the mouse serotonin receptor (5HT1c) cDNA in cultured insect cells].
| Autor: | Belzhelarskaia SN, Sattov F |
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| Jazyk: | ruština |
| Zdroj: | Molekuliarnaia biologiia [Mol Biol (Mosk)] 2004 May-Jun; Vol. 38 (3), pp. 477-82. |
| Abstrakt: | Baculovirus-mediated cloning and expression of the mouse serotonin receptor (5HT1c) cDNA in insect cells was proposed to obtain an alternative to an oocyte-based system, which is commonly employed in electrophysiological studies of ionic channels. A recombinant bacmid was constructed, and the 5HT1c cDNA transferred into the AcNPV genome to yield a recombinant baculovirus. Infected inset Sf9 cells produced recombinant 5HT1c. |
| Databáze: | MEDLINE |
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