| Autor: |
Mitiaeva ON, Nasedkina TV, Zharinov VS, Isaeva EA, Turygin AIu, Chupeeva VV, Kreĭndlin EIa, Mirzabekov AD |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2004 May-Jun; Vol. 38 (3), pp. 449-56. |
| Abstrakt: |
Identification of chromosome rearrangements is of importance for exact diagnosis, risk assessment, and therapy in blood malignancies. A new method was proposed for rapid and accurate identification of leukemia forms caused by chromosome rearrangements involving MLL (11q23). The method combines reverse transcription-multiplex PCR and hybridization with an oligonucleotide microarray. The microarray was designed to detect the five most common MLL rearrangements: t(4;11) MLL/AF4, t(9;11) MLL/AF9, t(11;19) MLL/ELL, t(11;19) MLL/ENL, and dup(11) MLL/MLL. With clinical specimens, the method was shown to efficiently identify the chromosome translocations in leukemia patients. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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