Alterations of the K-ras and p53 genes and microsatellite instability in sporadic colorectal carcinomas.

Autor:	Hasegawa A; Department of Surgery II, Fukushima Medical University, Fukushima 960-1295, Japan. hase@fmu.ac.jp, Fukushima T, Mashiko M, Nakano K, Suzuki S, Endo Y, Ohki S, Hatakeyama Y, Koyama Y, Inoue N, Takebayashi Y, Sekikawa K, Takenoshita S
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2004 May-Jun; Vol. 24 (3b), pp. 2047-52.
Abstrakt:	Background: The international guidelines for the evaluation of microsatellite instability (MSI) in colorectal cancer were defined in 1997 by the National Cancer Institute (NCI). Here, the relationship between MSI, cancer-associated genes and their clinicopathological variables were revaluated using these guidelines. Patients and Methods: Mutations of K-ras at exon 1 and p53 at exons 5, 6, 7 and 8 were analyzed in 43 cases of sporadic colorectal carcinoma. MSI was analyzed using the 5 markers recommended by the NCI reference panel. Results: The proportion of p53 mutations in the MSI-H cases (0 out of 5; 0%) was lower than that of non-MSI-H cases (23 out of 38; 60.5%) (p=0.0117). The proportion of p53 mutations in microsatellite stable (MSS) cases (21 out of 34; 61.8%) was higher than that of non-MSS cases (2 out of 9; 22.2%) (p=0.0366). The proportion of K-ras mutations in MSI-H tumors (1 out of 5; 20.0%) was less frequent than in non-MSI-H tumors (19 out of 38; 50.0%) (p=0.2115). Conclusion: p53 mutations in MSI-H tumors were much less common than in non-MSI-H tumors. This result suggested that alterations of the p53 gene are not closely associated with carcinogenesis in MSI-H carcinomas.
Databáze:	MEDLINE
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