The identification of genetically related bacterial isolates using pulsed field gel electrophoresis on nursing home units: a clinical experience.

Autor: Drinka PJ; Wisconsin Veterans Home, King, Wisconsin 54946, USA. Paul.Drinka@dva.state.wi.us, Stemper ME, Gauerke CD, Miller JM, Reed KD
Jazyk: angličtina
Zdroj: Journal of the American Geriatrics Society [J Am Geriatr Soc] 2004 Aug; Vol. 52 (8), pp. 1373-7.
DOI: 10.1111/j.1532-5415.2004.52371.x
Abstrakt: Objectives: To describe a laboratory-based technique to track nursing home infections.
Design: Retrospective data analysis.
Setting: A 721-bed skilled care facility with 14 nursing units.
Participants: Residents in a nursing home, average age 76+/-10, 78% male.
Measurements: Bacterial isolates were listed for each nursing unit. Clusters of identical species and antibiotic susceptibility were identified followed by pulsed-field gel electrophoresis (PFGE). If the genetic analysis yielded related strains, the director of nursing performed a clinical investigation. PFGE is available through reference laboratories at a cost of approximately 75 dollars/isolate.
Results: Twenty-four clinical clusters of phenotypically identical bacteria (species, antibiotic susceptibility) were identified. Fourteen included genetically related isolates.
Conclusion: Approximately half of the phenotypically identical clusters contained genetically related isolates. The identification of genetically related bacterial isolates on nursing units by PFGE provides staff with a specific circumstance to review secretion precautions. Genetic analysis may also demonstrate that apparent clusters are unrelated.
Databáze: MEDLINE
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