| Autor: |
Yoshisue H; Infection, Inflammation, and Repair Division, University of Southampton School of Medicine, Southampton General Hospital, Southampton, United Kingdom. hyoshisu@soton.ac.uk, Puddicombe SM, Wilson SJ, Haitchi HM, Powell RM, Wilson DI, Pandit A, Berger AE, Davies DE, Holgate ST, Holloway JW |
| Jazyk: |
angličtina |
| Zdroj: |
American journal of respiratory cell and molecular biology [Am J Respir Cell Mol Biol] 2004 Nov; Vol. 31 (5), pp. 491-500. Date of Electronic Publication: 2004 Jul 08. |
| DOI: |
10.1165/rcmb.2004-0050OC |
| Abstrakt: |
Lung epithelial structure is altered in asthma; however, the precise mechanisms underlying epithelial repair, including differentiation from basal to columnar epithelial cells, are not well defined. In the course of random sequencing of a cDNA library from human lung biopsies, we have identified a novel gene, ciliated bronchial epithelium 1 (CBE1). Expression of CBE1 was induced during in vitro differentiation of bronchial epithelial cells. Synchronous expression with tektin and hepatocyte nuclear factor 3/forkhead homologue 4, down-regulation by interleukin-13, and its tissue distribution strongly suggested that CBE1 is associated with ciliated cells. Two isoforms of the 0.7-kb full-length cDNA were identified, resulting in open reading frames with different carboxyl termini, with no homology to known proteins. Expression of CBE1 in ciliated epithelial cells was confirmed by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction analysis using bronchial biopsies showed no difference of expression of CBE1 between normal subjects and subjects with asthma. Expression studies showed that CBE1 is nuclear- or perinuclear-localized, depending on cell type. Regulated expression during differentiation and the subcellular localization of CBE1 suggest that it may play an important role in the differentiation and/or function of ciliated cells in human airways. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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