| Autor: |
Alexandrov A; Structural Genomics of Pathogenic Protozoa (SGPP) Consortium and Center for Human Genetics and Molecular Pediatric Disease, University of Rochester Medical Center, Rochester, NY 14642, USA., Vignali M, LaCount DJ, Quartley E, de Vries C, De Rosa D, Babulski J, Mitchell SF, Schoenfeld LW, Fields S, Hol WG, Dumont ME, Phizicky EM, Grayhack EJ |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2004 Sep; Vol. 3 (9), pp. 934-8. Date of Electronic Publication: 2004 Jul 07. |
| DOI: |
10.1074/mcp.T400008-MCP200 |
| Abstrakt: |
We developed a method to co-express protein pairs from collections of otherwise identical Escherichia coli plasmids expressing different ORFs by incorporating a 61-nucleotide sequence (LINK) into the plasmid to allow generation of tandem plasmids. Tandem plasmids are formed in a ligation-independent manner, propagate efficiently, and produce protein pairs in high quantities. This greatly facilitates co-expression for structural genomics projects that produce thousands of clones bearing identical origins and antibiotic markers. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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