| Autor: |
Verhelst SH; Leiden Institute of Chemistry, Gorlaeus Laboratories, P. O. Box 9502, 2300 RA Leiden, The Netherlands., Michiels PJ, van der Marel GA, van Boeckel CA, van Boom JH |
| Jazyk: |
angličtina |
| Zdroj: |
Chembiochem : a European journal of chemical biology [Chembiochem] 2004 Jul 05; Vol. 5 (7), pp. 937-42. |
| DOI: |
10.1002/cbic.200300819 |
| Abstrakt: |
Aminoglycoside antibiotics, which are able to selectively bind to RNA, are considered to be an important lead in RNA-targeting drug discovery. In this study, surface plasmon resonance (SPR) was employed to explore the interaction of aminoglycosides with known tobramycin-binding RNA hairpins (aptamers) and an unrelated RNA hairpin. It was established that aminoglycosides have multiple interactions with RNA hairpins. Unexpectedly, the different hairpins showed comparable affinity for a set of related aminoglycosides. The observed absence of selectivity presents an extra hurdle in the discovery of novel aminoglycosides as specific drugs that target defined RNA hairpins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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