| Autor: |
Hassepass I; Cell Cycle Control and Carcinogenesis, German Cancer Research Center (DKFZ), Heidelberg., Hoffmann I |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2004; Vol. 281, pp. 153-62. |
| DOI: |
10.1385/1-59259-811-0:153 |
| Abstrakt: |
To determine the activity of all human Cdc25 phosphatases, two different methods are described. For assaying phosphatase activities of recombinant Cdc25 proteins produced in Escherichia coli or insect cells, a fluorimetric assay using fluorescein diphosphate (FDP) as a substrate is recommended. To analyze endogenous Cdc25 phosphatase activities of immunoprecipitates from total cellular extracts, the physiological substrate Cdk1/cyclin B1 is most sensitive. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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