| Autor: |
Hornbaek T; Novozymes A/S, Bagsvaerd, Denmark. tho@kvl.dk, Nielsen AK, Dynesen J, Jakobsen M |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS microbiology letters [FEMS Microbiol Lett] 2004 Jul 01; Vol. 236 (1), pp. 145-51. |
| DOI: |
10.1016/j.femsle.2004.05.035 |
| Abstrakt: |
Shorter lag phases were obtained in cultivations of Bacillus licheniformis using early-compared to late-stationary growth phase inocula and using liquid versus solid propagation medium. Flow cytometry and fluorescence ratio imaging microscopy (FRIM) after staining with 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), confirmed that liquid early-stationary growth phase inoculum had a higher vitality and was more homogeneous than solid late-stationary growth phase inoculum. DNA-microarray analyses indicated that liquid early-stationary growth phase inoculum was in a more active state in terms of cell multiplication whereas solid late-stationary growth phase inoculum was induced to some spore formation potentially causing delayed growth initiation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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