[The expression and activity detection of a variant N protein of SARS-CoV].

Autor:	Qi Y; Department of Immunology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. qiy2004@sohu.com, Zheng Y, Shu CL, Jiang L, Hu Y, Mao PY, Cheng Y
Jazyk:	čínština
Zdroj:	Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2004 Jul; Vol. 20 (4), pp. 422-4.
Abstrakt:	Aim: To construct an expression vector pGEX-2T/N, and to express the fusion protein consisting of N protein of SARS-CoV and GST in E.coli. Methods: The N region gene of SARS-CoV was cloned by RT-PCR. The expression vector was constructed by DNA recombination. The recombinant plasmid was transformed into E.coli BL21(DE3). The expression of the fusion protein was detected by Western blot. Results: (1) As compared with the sequences in GenBank, 20 bp were deleted in DNA sequence of the cloned N protein. (2) The fusion protein GST-N was soluble. Western blot analysis showed that the reaction of GST-N to anti-SARS-CoV sera was positive. Conclusion: The pGEX-2T/N has been constructed and expressed in the form of fusion protein GST-N successfully, which lays the foundation for further study of SARS-CoV N protein.
Databáze:	MEDLINE
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