Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development.
| Autor: | Imamura T; INSERM E367, Institut Jacques Monod; 2, place Jussieu, 75005 Paris France. imamura@nibb.ac.jp, Neildez TM, Thenevin C, Paldi A |
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| Jazyk: | angličtina |
| Zdroj: | BMC molecular biology [BMC Mol Biol] 2004 Jun 16; Vol. 5, pp. 4. Date of Electronic Publication: 2004 Jun 16. |
| DOI: | 10.1186/1471-2199-5-4 |
| Abstrakt: | Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote. |
| Databáze: | MEDLINE |
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