| Autor: |
Ivanov VS; Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of Russia, Moscow., Suvorova ZK, Tchikin LD, Kozhich AT, Ivanov VT |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1992 Aug 30; Vol. 153 (1-2), pp. 229-33. |
| DOI: |
10.1016/0022-1759(92)90326-o |
| Abstrakt: |
A procedure is described for the immobilization of synthetic peptide antigens on a plastic solid phase for performing ELISA. The use of a streptavidin-biotinylated peptide system for coating microplates with peptide antigen markedly increased both the sensitivity and the specificity compared to a standard ELISA based on synthetic peptides. The procedure was used for the detection of HIV-1-specific antibodies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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