Polyethylene glycol-grafted polystyrene particles.

Autor: Meng F; Polymer Chemistry and Biomaterials Group, Department of Chemical Technology, Institute for Biomedical Technology (BMTI), University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands., Engbers GH, Feijen J
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2004 Jul 01; Vol. 70 (1), pp. 49-58.
DOI: 10.1002/jbm.a.30056
Abstrakt: Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, zeta-potential, size, and morphology. Under optimized grafting conditions, a dense "brush-like" PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm2, corresponding with an average distance between grafted PEG chains of approximately 17 A can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%.
(Copyright 2004 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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