The colon mitosis inhibitor pyroglutamyl-histidyl-glycine inhibits growth of non-tumorigenic colonic epithelial cells.

Autor:	Reichelt WH; Institute of Pediatric Research, University of Oslo, The National Hospital, NO-0027 Oslo, Norway. w.h.reichelt@klinmed.uio.no, De Angelis PM, Knutsen HK, Husøy T, Elgjo K, Reichelt KL
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2004 Mar-Apr; Vol. 24 (2B), pp. 587-91.
Abstrakt:	Background: The colon mitosis inhibiting peptide pyroglutamyl-histidyl-glycine (pEHG) increases the expression of c-fos, fosB and egr-1 genes in the colon carcinoma cell line HT-29. However, the effect on non-tumorigenic colonic cells has not been investigated. Materials and Methods: After exposure of the cell lines YAMC (from colon mucosa of Immorto mice) and IMCE (fromn Immorto-Min mouse hybrid) to pEHG, DNA-synthesis was analysed by H3-thymidine incorporation, apoptosis and necrosis by fluorescence microscopy, and cell cycle distribution by flow cytometry. Results: pEHG inhibited DNA-synthesis with a maximal effect at 10(-8)-10(-9) M, but stimulated at 10(-4) M. It blocked cell flow through the cell cycle at GC/M after 8 h of treatment, but had no effect on apoptosis or necrosis at any concentration. A low concentration of ascorbic acid stabilised the cells, maybe as a free radical scavanger. Conclusion: pEHG inhibits flux at the G2/M transition, but has no effect on cell death.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu