| Abstrakt: |
A new technology of deriving the whole-culture peroral meningococcal mono-vaccines of serogroups A, B and C from the acetone-inactivated cultures was experimentally substantiated. The latter cultures were obtained through computer-aided continuous-flow cultivation of Neisseria meningitides of the above serogroups in the bioreactor with a synthetic nutrient medium. The whole-culture meningococcal mono-preparation of 3 serogroups comprised a complete set of antibodies, i.e. polysaccharides (PS), external membrane proteins (EMP) and lipopolysaccharides (LPP), in their native condition. High levels of IgG to PS, EMP and LPP were detected in blood sera of rabbits, which had been perorally immunized by the above preparations; such high levels persisted for a long time (303 days--observation period). It is noteworthy, that in accumulating of IgG to all investigated antigens of meningococcus of serogroups A and C, the optimal doses of whole-culture peroral monovaccines did not exceed the culture doses used to derive the hyper-immune sera at intravenous immunization. |
