| Autor: |
Stedman DR; Department of Psychiatry, Yale University School of Medicine, 34 Park Street - 3rd Floor Research, New Haven, CT 05608, USA., Uboha NV, Stedman TT, Nairn AC, Picciotto MR |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 2004 May 21; Vol. 566 (1-3), pp. 275-80. |
| DOI: |
10.1016/j.febslet.2004.04.042 |
| Abstrakt: |
Calcium/calmodulin-dependent protein kinase I-alpha (CaMKI-alpha) is a ubiquitous cytosolic enzyme that phosphorylates a number of nuclear proteins in vitro and has been implicated in transcriptional regulation. We report that cytoplasmic localization of CaMKI-alpha depends on CRM1-mediated nuclear export mediated through a Rev-like nuclear export signal in the CaMKI-alpha regulatory domain. Interaction of CaMKI-alpha with a CRM1 complex in vitro is enhanced by incubation with calcium/calmodulin. Translocation of CaMKI-alpha into the nucleus involves a conserved sequence located within the catalytic core. Mutation of this sequence partially blocks nuclear entry of an export-impaired mutant of CaMKI-alpha. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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