| Autor: |
Chirara MM; Department of Biochemistry, University of Zimbabwe, Mount Pleasant, Harare., Chetsanga CJ |
| Jazyk: |
angličtina |
| Zdroj: |
Scandinavian journal of immunology. Supplement [Scand J Immunol Suppl] 1992; Vol. 11, pp. 63-6. |
| DOI: |
10.1111/j.1365-3083.1992.tb01621.x |
| Abstrakt: |
The polymerase chain reaction (PCR) was used to amplify the pre-S1, pre-S2 and S gene regions of hepatitis B virus (HBV). Sera from three different patients were used as the source of HBV DNA. The resulting 1.2-kb amplification product was cloned into the plasmid pIBI30. Restriction enzyme analysis revealed that there are two BamHI sites located about 300 bp apart within the S gene. DNA sequencing revealed a greatest homology to the HBVadw subtype. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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