Role of Ca2+ in induction of neurotransmitter-related gene expression by butyrate.
| Autor: | Zia MT; Division of Newborn Medicine Westchester Medical Center/Department of Pediatrics; Department of Biochemistry and Molecular Biology, The Regional Neonatal Center, New York Medical College-Westchester Medical Center, Valhalla, NY 10595, USA., Nankova BB, Krishnan K, Gueorguiev VD, Frenz CM, Sabban EL, La Gamma EF |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Neuroreport [Neuroreport] 2004 May 19; Vol. 15 (7), pp. 1177-81. |
| DOI: | 10.1097/00001756-200405190-00019 |
| Abstrakt: | We examined the effect of butyrate on neurotransmitter-related gene expression and calcium homeostasis in PC12 cells. Pretreatment with Ca2+ chelators (EGTA or BAPTA-AM) attenuated the butyrate-triggered accumulation of TH and ppEnk mRNA indicating that Ca2+ plays a role in butyrate-induced regulation of neuronal genes. Butyrate alone did not alter intracellular Ca2+ levels as determined by Fura-PE3 fluorescence; however, pretreatment with butyrate (18-24 h) reduced the first Ca2+ peak and prevented the second sustained rise in [Ca2+]i as induced by nicotine or ryanodine. In contrast, butyrate had no effect on Ca2+ transients when added shortly before or during nicotine or ryanodine stimulation. These results suggest that chronic butyrate exposure can modulate cell responses by affecting intracellular Ca2+ signaling. (Copyright 2004 Lippincott Williams and Wilkins) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|