| Autor: |
Fan JB; Illumina, Inc., San Diego, California 92121, USA. jfan@illumina.com, Yeakley JM, Bibikova M, Chudin E, Wickham E, Chen J, Doucet D, Rigault P, Zhang B, Shen R, McBride C, Li HR, Fu XD, Oliphant A, Barker DL, Chee MS |
| Jazyk: |
angličtina |
| Zdroj: |
Genome research [Genome Res] 2004 May; Vol. 14 (5), pp. 878-85. |
| DOI: |
10.1101/gr.2167504 |
| Abstrakt: |
We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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