| Autor: |
Penfold RJ; Department of Microbiology, University of Queensland, St. Lucia, Australia., Pemberton JM |
| Jazyk: |
angličtina |
| Zdroj: |
Gene [Gene] 1992 Sep 01; Vol. 118 (1), pp. 145-6. |
| DOI: |
10.1016/0378-1119(92)90263-o |
| Abstrakt: |
We have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of the pir-encoded pi protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it has nine unique restriction sites available for alpha-complementation cloning. Vector functionality was demonstrated in Rhodobacter sphaeroides. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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