Molecular cloning, chromosomal location, and biological activity of porcine interleukin-21.
| Substance Nomenclature: | 0 (DNA Primers) 0 (DNA, Complementary) 0 (Interleukins) 82115-62-6 (Interferon-gamma) MKM3CA6LT1 (interleukin-21) |
|---|---|
| Entry Date(s): | Date Created: 20040427 Date Completed: 20040521 Latest Revision: 20190605 |
| Update Code: | 20231215 |
| DOI: | 10.1292/jvms.66.269 |
| PMID: | 15107555 |
| Autor: | Muneta Y; National Institute of Animal Health, Tsukuba, Ibaraki, Japan., Kikuma R, Uenishi H, Hoshino T, Yoshihara K, Tanaka M, Hamashima N, Mori Y |
| Jazyk: | angličtina |
| Zdroj: | The Journal of veterinary medical science [J Vet Med Sci] 2004 Mar; Vol. 66 (3), pp. 269-75. |
| DOI: | 10.1292/jvms.66.269 |
| Abstrakt: | A pig interleukin-21 (IL-21) cDNA was successfully cloned and sequenced from porcine peripheral blood lymphocytes (PBL) stimulated with 10 microg/ml concanavalin A (ConA), 10 microg/ml phytohemagglutinin P (PHA), 50 ng/ml phorbol 12-myristate 13-acetate (PMA), and 0.5 microg/ml anti-porcine CD3 antibody for 48 hr. The open reading frame of the porcine IL-21 cDNA is 459 base pairs in length and encodes 152 amino acids. The predicted amino acid sequence of the porcine IL-21 shows 86.2%, 77.7%, and 58.4% identity to the bovine, human, and murine IL-21, respectively. The porcine IL-21 gene was mapped to porcine chromosome 8 (8q22-->q23) by means of fluorescence in situ hybridization and radiation hybrid mapping, where the porcine IL-2 gene had been mapped nearby. The recombinant porcine mature IL-21 expressed by E. coli induced dose-dependent proliferation and IFN-gamma production from a human NK cell line, NK0. The porcine IL-21 identified in this study will be helpful for the enhancement of innate immune responses of pigs. |
| Databáze: | MEDLINE |
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