| Autor: |
Almeida RS; Laboratório de Virologia Animal, Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas-UNICAMP, 13081-970 Campinas, SP, Brazil., Domingues HG, Coswig LT, D'Arce RC, de Carvalho RF, Arns CW |
| Jazyk: |
angličtina |
| Zdroj: |
Veterinary research [Vet Res] 2004 Mar-Apr; Vol. 35 (2), pp. 189-97. |
| DOI: |
10.1051/vetres:2004003 |
| Abstrakt: |
The present study used an RT-nested-PCR and an immunohistochemistry assay to detect bovine respiratory syncytial virus in tissues from experimentally infected balb/c mice. As a first step, Chicken Embryo Related (CER) cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. Then, the infected lung and tracheal tissues of female balb/c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. Primers specific to F and G genes that amplify fragments of 481 bp and 371 bp, respectively, were used. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment. This was probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Moreover, only three of the lungs collected five days post-infection were positive by immunohistochemistry. To the author's knowledge, this is the first study reporting bovine respiratory syncytial virus detection in balb/c mice after experimental inoculation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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