Immunomodulatory effects of sucupira (Pterodon pubescens) seed infusion on collagen-induced arthritis.
| Autor: | Coelho MG; Department of Biochemistry, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil., Sabino KC, Dalmau SR |
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| Jazyk: | angličtina |
| Zdroj: | Clinical and experimental rheumatology [Clin Exp Rheumatol] 2004 Mar-Apr; Vol. 22 (2), pp. 213-8. |
| Abstrakt: | Objective: To evaluate the clinical and immunomodulatory efficacy of seed extracts from sucupira branca (Pterodon pubescens Benth.), a Brazilian anti-inflammatory folk medicine, against collagen II (CII)-induced arthritis (CIA) in mice. Methods: Mice were divided into 3 groups: 1) normal control mice received a vehicle (ethanol 15% in water); 2) mice with CIA received the vehicle; and 3) mice with CIA received extract from 1 mg sucupira seeds/day. The daily oral treatments started 21 days after the first collagen immunization, ending 4 weeks later. We analyzed the arthritic index, the histopathology of the joints, the serum anti-CII IgG antibody level, and the absolute counts of the CD4+, CD8+, CD4+CD69+ and CD8+CD69+ subsets of inguinal lymph nodes (LN). Results: Sucupira treatment strongly reduced the severity of arthritis (p < 0.001). Vehicle-treated CIA mice exhibited invasive synovial pannus and significant articular leukocyte infiltration, features that were reduced or absent in sucupira-treated mice. Mice with CIA exhibited twice the number of CD4+ and CD8+ LN cells found in control mice. Suctupira-treated mice exhibited these subsets in numbers comparable to the latter. A two-thirds decrease in the level of serum anti-CII IgG antibody and a normalization of the CD4+CD69+ LN cell number in treated mice hallmark a negative regulatory effect of sucupira on B- and CD4 T-cell activation, respectively. The CD8+CD69+ cell number remained roughly the same in the 3 groups. Conclusion: The clinical and immunomodulatory effects of sucupira on CIA provides a further experimental basis for the popular use of sucupira in chronic inflammatory diseases. |
| Databáze: | MEDLINE |
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