| Autor: |
Kroutil W; Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Austria., Mang H, Edegger K, Faber K |
| Jazyk: |
angličtina |
| Zdroj: |
Current opinion in chemical biology [Curr Opin Chem Biol] 2004 Apr; Vol. 8 (2), pp. 120-6. |
| DOI: |
10.1016/j.cbpa.2004.02.005 |
| Abstrakt: |
To improve the efficiency and applicability of biocatalytic redox-reactions for asymmetric ketone-reduction and enantioselective alcohol-oxidation catalyzed by nicotinamide-dependent dehydrogenases/reductases, several achievements for cofactor-recycling have been made during the last two years. First, the use of hydrogenases for NADPH recycling in a two enzyme system. Second, preparative transformations with alcohol dehydrogenases coupled with NADH oxidases for NAD+/NADP+ recycling. Third, an exceptional chemo-stable alcohol dehydrogenase can efficiently use i-propanol and acetone as cosubstrates for reduction and oxidation, respectively, in a single-enzyme system. Novel carbonyl reductases and dehydrogenases derived from plant cells are particularly suited for sterically demanding substrates. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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