| Autor: |
Roy AC; Department of Obstetrics and Gynaecology, National University of Singapore., Saha N, Tan SM, Kamarul FZ, Ratnam SS |
| Jazyk: |
angličtina |
| Zdroj: |
Electrophoresis [Electrophoresis] 1992 Jun; Vol. 13 (6), pp. 396-7. |
| DOI: |
10.1002/elps.1150130181 |
| Abstrakt: |
A sensitive staining method for the detection of oxytocinase (EC 3.4.11.3) activity in electrophoresis gels has been described. The method is based on the enzymatic release of p-nitroaniline (PNA) from two specific synthetic oxytocinase substrates, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), respectively. The PNA is then diazotized with sodium nitrite and subsequently coupled to a chromogen, N-(1-naphthyl)-ethylenediamine dihydrochloride (NED) to produce a deep pink/magenta colored azo-dye at the site of oxytocinase activity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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