| Autor: |
Duta FP; Universidade Federal do Rio de Janeiro, Departamento de Engenharia Bioquímica, Bloco E, Ilha do Fundão, Rio de Janeiro, RJ CEP: 21949-900 Brazil., De França FP, Sérvulo EF, De Almeida Lopes LM, Da Costa AC, Barros A |
| Jazyk: |
angličtina |
| Zdroj: |
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2004 Spring; Vol. 113-116, pp. 639-52. |
| DOI: |
10.1385/abab:114:1-3:639 |
| Abstrakt: |
The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic of microbially produced biopolymers, including C = O and O-acetyl groups. Thermogravimetric analysis showed the characteristic degradation profiles of the exopolysaccharide produced (T(onset) = 290degreesC). The experimental design showed that a low substrate concentration (10.0 g/L), and low aeration (0.2 vvm) and agitation (200 rpm) levels should be used. The maximum yield of the process was a Yp/s (g/g) of 0.19 +/- 0.1, obtained under optimized conditions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink