| Autor: |
Petach H; SomaLogic Inc, Boulder, CO, USA., Ostroff R, Greef C, Husar GM |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2004; Vol. 264, pp. 101-10. |
| DOI: |
10.1385/1-59259-759-9:101 |
| Abstrakt: |
Photoaptamers are single-stranded nucleic acids selected for their high affinity to specific proteins of interest. Photoaptamer microarrays capture and quantify proteins from complex samples using a unique protocol that leverages both high-affinity capture with covalent retention of analytes. The initial capture of proteins from solution is similar to the well-known antibody capture, but the "secondary binding event" affected by photoaptamers is a covalent crosslink between the photoaptamer capture agent and the protein analyte. The nature of this specific covalent reaction allows a unique microarray processing that is described in detail in this chapter. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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