| Autor: |
Maritim AC; Protozoology Division, National Veterinary Research Centre, Muguga, Kenya., Young AS, Lesan AC, Ndungu SG, Stagg DA, Ngumi PN |
| Jazyk: |
angličtina |
| Zdroj: |
Veterinary parasitology [Vet Parasitol] 1992 Jun; Vol. 43 (1-2), pp. 1-14. |
| DOI: |
10.1016/0304-4017(92)90043-9 |
| Abstrakt: |
A sporozoite stabilate (St. 199) of Theileria parva was obtained by feeding nymphal Rhipicephalus appendiculatus on an African buffalo (Syncerus caffer) and was used to immunize cattle by the infection and treatment method. Nymphal ticks were applied to one of the steers 90 days later and it was shown that the resultant adult tick had become infected. Using tick/cattle passage, two passage lines of T. parva were established. By the fifth tick/cattle passage, the parasite stocks had changed their behaviour to that of T. parva derived from cattle as the parasite produced relatively high schizont parasitosis and piroplasm parasitaemia in cattle, and had become highly infective to ticks. At various passage levels the parasite populations were characterized by behaviour and by monoclonal antibodies against T. parva schizonts using infected cell culture isolates from cattle during acute infections. The monoclonal antibody profile showed little evidence of antigen change of the parasite during passage through cattle, which was confirmed in a two-way cross-immunity experiment using sporozoite stabilate derived from ticks obtained from the buffalo and fourth passage in cattle. The implication of these results, particularly in relationship to immunization of cattle against T. parva derived from buffalo, is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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