| Autor: |
Kit IuIa; Institute of Cell Biology, NAS of Ukraine, Lviv. kit@biochem.lviv.ua, Koval'ova VA, Bobak IaP, Drobot LB |
| Jazyk: |
ukrajinština |
| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2002 Jul-Aug; Vol. 74 (4), pp. 129-32. |
| Abstrakt: |
Method for detection of protein kinase activity in polyacrylamide gel have been developed. After separation of proteins by isoelectric focusing in non-denaturing condition, gel was incubated in a reaction buffer containing [gamma-32P]ATP. 32P-labeled proteins were separated by subsequent SDS/PAGE electrophoresis in second dimension. The proposed method was used for detection of protein kinase activity in human blood serum and triton X-100 soluble proteins of heads of Drosophila melanogaster. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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