| Autor: |
Boltovets PN; Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv. pboltovec@mail.ru, Verevka SV, Diachenko NS, Snopok BA, Shirshov IuM |
| Jazyk: |
ruština |
| Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2002 Jul-Aug; Vol. 74 (4), pp. 71-5. |
| Abstrakt: |
When studying biospecific interactions with application of surface plasmon resonance, one of the main problems is reagent proper orientation to the sensor surface. Due to rather high chemical activity of molecular receptor sites, the interaction between these areas and surface may become predominant. Here we propose a technique for prevention of such orientation of bioreceptors using soybean trypsin inhibitor STI as an example. To obtain oriented STI immobilization on a modified gold surface its active site has been previously blocked through interaction with its specific partner trypsin. After conjugate immobilization on the sensor surface the components were separated using a glycine buffer (pH 2.2). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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