A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies.

Autor: Moratelli R; Biologia Animal, UF Rural RJ, BR 465, km 07, 23890-000 Seropédica, RJ, Brasil. rimoratelli@bol.com.br, Andrade Cde M, de Armada JL
Jazyk: angličtina
Zdroj: Genetics and molecular research : GMR [Genet Mol Res] 2002 Jun 30; Vol. 1 (2), pp. 128-30. Date of Electronic Publication: 2002 Jun 30.
Abstrakt: We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary cultures can be used for other biological studies. This procedure does not require sacrificing the animals.
Databáze: MEDLINE