| Autor: |
Dzikowski R; Department of Animal Sciences, Faculty of Agriculture, Food and Environmental Sciences, the Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel. rrd2001@med.cornell.edu, Levy MG, Poore MF, Flowers JR, Paperna I |
| Jazyk: |
angličtina |
| Zdroj: |
Diseases of aquatic organisms [Dis Aquat Organ] 2003 Dec 29; Vol. 57 (3), pp. 231-5. |
| DOI: |
10.3354/dao057231 |
| Abstrakt: |
Metacercariae of Bolbophorus species are serious pathogens of farmed fish. Molecular diagnostic tools, capable of identifying and differentiating these parasites, may assist in the development of rationale control strategies. The rDNA 18S (small sub-unit: SSU) genes of adult B. confusus and B. levantinus obtained from a pelican, Pelecanus onocrotalus, and a night heron, Nycticorax nycticorax, respectively, were amplified, sequenced, and aligned. Based on this alignment, we developed a genetic differentiation assay between B. confusus and B. levantinus. These 2 species were compared genetically with the North American species B. damnificus and Bolbophorus sp. ('Type 2'). The relationship between species is outlined and discussed. In addition to the molecular study, specimens of B. confusus and B. levantinus were compared morphologically, using scanning electron microscopy. Morphologic analysis revealed interspecific differences in details of the holdfast organ and the position of the acetabulum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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