| Autor: |
Zheng YL; Department of Pathology, University of Cambridge, U.K., Ferguson-Smith MA, Warner JP, Ferguson-Smith ME, Sargent CA, Carter NP |
| Jazyk: |
angličtina |
| Zdroj: |
Prenatal diagnosis [Prenat Diagn] 1992 Nov; Vol. 12 (11), pp. 931-43. |
| DOI: |
10.1002/pd.1970121113 |
| Abstrakt: |
A comparison of the use of chromosome 21-specific libraries, DOP-PCR 21 paints, yeast artificial chromosome (YAC) clones, single cosmids, and a 21q cosmid contig as probes for the detection of the copy number of chromosome 21 in interphase cells by fluorescence in situ hybridization shows that the cosmid contig is a satisfactory probe for interphase analysis of chromosome 21. The contig cCMP21.a, which is 55 kb in length, is highly chromosome 21-specific and produces intense, compact signals in a high proportion of interphase cells. A retrospective blind analysis of coded uncultured amniotic fluid samples correctly detected four trisomy 21 cases out of 49 samples. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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