| Autor: |
Budd TJ; Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham, UK., Dolman CD, Lawson AM, Chai W, Saxton J, Hemming FW |
| Jazyk: |
angličtina |
| Zdroj: |
Glycoconjugate journal [Glycoconj J] 1992 Oct; Vol. 9 (5), pp. 274-8. |
| DOI: |
10.1007/BF00731139 |
| Abstrakt: |
N-Acetylneuraminic acid (Neu5Ac) and N-glycoloylneuraminic acid (Neu5Gc) are distributed widely in nature. Using a Carbopac PA-1 anion exchange column, we have determined the ratios of Neu5Ac and Neu5Gc in hydrolysates of platelets and their precursors: a rat promegakaryoblastic (RPM) cell line and a human megakaryoblastic leukemia cell line (MEG-01). The ratio of Neu5Gc:Neu5Ac in cultured RPM cells is 16:1, whereas in platelet rich plasma and cultured MEG-01 cells it is 1:38 and 1:28, respectively. The nature of these sialic acids from RPM cells was verified using thin layer chromatography and liquid secondary ion mass spectrometry. The relevance of increased Neu5Gc levels in early stages of development is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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