| Autor: |
Hawkins AL; Johns Hopkins Oncology Center, Baltimore, MD 21205., Jones RJ, Zehnbauer BA, Zicha MS, Collector MJ, Sharkis SJ, Griffin CA |
| Jazyk: |
angličtina |
| Zdroj: |
Cancer genetics and cytogenetics [Cancer Genet Cytogenet] 1992 Dec; Vol. 64 (2), pp. 145-8. |
| DOI: |
10.1016/0165-4608(92)90345-9 |
| Abstrakt: |
The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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