| Autor: |
Casareale D; Roche Molecular Systems, Branchburg, New Jersey 08876-1760., Pottathil R, Diaco R |
| Jazyk: |
angličtina |
| Zdroj: |
PCR methods and applications [PCR Methods Appl] 1992 Nov; Vol. 2 (2), pp. 149-53. |
| DOI: |
10.1101/gr.2.2.149 |
| Abstrakt: |
To simplify our procedure for blood sample processing for PCR, we introduced a simpler, shorter, and more cost-effective method for the separation of peripheral blood lymphocytes (PBL) and DNA extraction for amplification with Taq polymerase. By this method, blood samples are processed in two simple 15-min steps: (1) separation of PBLs from whole blood by red blood cell lysis with the Roche Specimen Washing Solution, and (2) DNA extraction by heat-detergent treatment of separated PBLs. This new method is simpler than the standard Ficoll-Hypaque method for PBLs separation and Proteinase K digestion for DNA extraction. It is not inhibitory to DNA amplification and it allows effective processing of blood samples even after prolonged storage (as long as 8 days) at room temperature. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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