| Autor: |
Shi N; National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, National Human Genome Center, Beijing 100005, People's Republic of China., Liu Y, Ni M, Yang M, Wu J, Peng Y, Gao F, Sun F, Peng X, Qiang B, Rao Z, Yuan J |
| Jazyk: |
angličtina |
| Zdroj: |
Acta crystallographica. Section D, Biological crystallography [Acta Crystallogr D Biol Crystallogr] 2004 Feb; Vol. 60 (Pt 2), pp. 334-6. Date of Electronic Publication: 2004 Jan 23. |
| DOI: |
10.1107/S0907444903026696 |
| Abstrakt: |
The PTB domain of mouse dok1 fusion protein has been overexpressed in Escherichia coli and crystallized in a form suitable for X-ray crystallographic study. Crystals have been obtained using the vapour-diffusion method and belong to space group P2(1)2(1)2(1). X-ray diffraction data were collected in-house to 2.5 A resolution. A selenomethionine (SeMet) dok1 PTB fusion-protein derivative was expressed using the same expression system, purified in a reductive environment and crystals were obtained under similar conditions. Subsequently, three different wavelength data sets from the derivative crystal were collected to 2.5 A resolution at SPring-8. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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