| Autor: |
Sousa-Majer MJ; Department of Environmental Biology, Curtin University of Technology PO Box U1987, Perth, WA 6845, Australia. M.Majer@curtin.edu.au, Turner NC, Hardie DC, Morton RL, Lamont B, Higgins TJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of experimental botany [J Exp Bot] 2004 Feb; Vol. 55 (396), pp. 497-505. Date of Electronic Publication: 2004 Jan 12. |
| DOI: |
10.1093/jxb/erh037 |
| Abstrakt: |
The effects of water deficit and high temperature on the production of alpha-amylase inhibitor 1 (alpha-AI-1) were studied in transgenic peas (Pisum sativum L.) that were developed to control the seed-feeding pea weevil (Bruchus pisorum L., Coleoptera: Bruchidae). Transgenic and non-transgenic plants were subjected to water-deficit and high-temperature treatments under controlled conditions in the glasshouse and growth cabinet, beginning 1 week after the first pods were formed. In the water-deficit treatments, the peas were either adequately watered (control) or water was withheld after first pod formation. The high-temperature experiments were performed in two growth cabinets, one maintained at 27/22 degrees C (control) and one at 32/27 degrees C day/night temperatures, with the vapour pressure deficit maintained at 1.3 kPa. The plants exposure to high temperatures and water deficit produced 27% and 79% fewer seeds, respectively, than the controls. In the transgenic peas the level of alpha-AI-1 as a percentage of total protein was not influenced by water stress, but was reduced on average by 36.3% (the range in two experiments was 11-50%) in the high-temperature treatment. Transgenic and non-transgenic pods of plants grown at 27/22 degrees C and 32/27 degrees C were inoculated with pea weevil eggs to evaluate whether the reduction in level of alpha-AI-1 in the transgenic pea seeds affected pea weevil development and survival. At the higher temperatures, 39% of adult pea weevil emerged, compared to 1.2% in the transgenic peas grown at the lower temperatures, indicating that high temperature reduced the protective capacity of the transgenic peas. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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