Fluid mechanics of a spinner-flask bioreactor.

Autor: Sucosky P; Georgia Institute of Technology, The George W Woodruff School of Mechanical Engineering, 771 Ferst Drive, Atlanta, Georgia 30332-0405, USA., Osorio DF, Brown JB, Neitzel GP
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2004 Jan 05; Vol. 85 (1), pp. 34-46.
DOI: 10.1002/bit.10788
Abstrakt: Spinner-flask bioreactors have been used for the production of articular cartilage in vitro. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. The present research focuses on the experimental and numerical characterization of the flow field within a spinner flask operating under conditions used to produce cartilage. Laboratory experiments carried out in a scaled-up model bioreactor employ particle-image velocimetry (PIV) to determine velocity and shear-rate fields in the vicinity of the construct closest to the stir bar, in addition to turbulence properties. Numerical computations calculated using FLUENT, a commercial software package, simulate the flow field in the same model bioreactor under similar operating conditions. In the computations, scaffolds were modeled as both solid and porous media with different permeabilities and flow rates through various faces of the construct nearest the stir bar were examined.
(Copyright 2003 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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