| Autor: |
Checroun C; Laboratoire de Microbiologie et Génétique Moléculaire, UMR5100 CNRS-Université Toulouse III, 118, Route de Narbonne, 31062, Toulouse Cedex, France., Bordes P, Leroy O, Kolb A, Gutierrez C |
| Jazyk: |
angličtina |
| Zdroj: |
Nucleic acids research [Nucleic Acids Res] 2004 Jan 02; Vol. 32 (1), pp. 45-53. Date of Electronic Publication: 2004 Jan 02 (Print Publication: 2004). |
| DOI: |
10.1093/nar/gkh155 |
| Abstrakt: |
The sigmas subunit of Escherichia coli RNA polymerase holoenzyme (EsigmaS) is a key factor of gene expression upon entry into stationary phase and in stressful conditions. The selectivity of promoter recognition by EsigmaS and the housekeeping Esigma70 is as yet not clearly understood. We used a genetic approach to investigate the interaction of sigmaS with its target promoters. Starting with down-promoter variants of a sigmaS promoter target, osmEp, altered in the -10 or -35 elements, we isolated mutant forms of sigmaS suppressing the promoter defects. The activity of these suppressors on variants of osmEp and ficp, another target of sigmaS, indicated that sigmaS is able to interact with the same key features within a promoter sequence as sigma70. Indeed, (i) sigmaS can recognize the -35 element of some but not all its target promoters, through interactions with its 4.2 region; and (ii) amino acids within the 2.4 region participate in the recognition of the -10 element. More specifically, residues Q152 and E155 contribute to the strong preference of sigmaS for a C in position -13 and residue R299 can interact with the -31 nucleotide in the -35 element of the target promoters. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
