[Effect of spermine on activity of purified Ca2+, Mg2+-ATPase from plasma membranes of myometrium cells].

Autor: Slinchenko NM; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. slinchenko@biochem.kiev.ua, Chernysh IH, Kosterin SO
Jazyk: ukrajinština
Zdroj: Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2003 Jul-Aug; Vol. 75 (4), pp. 51-6.
Abstrakt: Effect of endogenous polyamine spermine, a relaxant of smooth muscle, on the activity of myometrium cell plasma membrane Ca2+, Mg(2+)-ATPase was studied. It was observed a tendency to activation of enzyme at the spermine concentrations 0.1-0.5 mM, the increase of the polyamine concentrations up to 10 mM inhibited. ATPase by 80% (I50 = 5.5 +/- 0.3 mM). Spermine inhibited enzyme decreasing its turnover rate and affinity for Ca2+. The ATPase affinity for Mg2+ increased in the presence of spermine. It was revealed, that the inhibitory effect of spermine is changed by the stimulatory effect under the increase of Ca2+ concentration (up to 2.6 microM), that correlates with the relaxing effect of this polyamine on the smooth muscle.
Databáze: MEDLINE