Autor: |
Slinchenko NM; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. slinchenko@biochem.kiev.ua, Chernysh IH, Kosterin SO |
Jazyk: |
ukrajinština |
Zdroj: |
Ukrains'kyi biokhimichnyi zhurnal (1999 ) [Ukr Biokhim Zh (1999)] 2003 Jul-Aug; Vol. 75 (4), pp. 51-6. |
Abstrakt: |
Effect of endogenous polyamine spermine, a relaxant of smooth muscle, on the activity of myometrium cell plasma membrane Ca2+, Mg(2+)-ATPase was studied. It was observed a tendency to activation of enzyme at the spermine concentrations 0.1-0.5 mM, the increase of the polyamine concentrations up to 10 mM inhibited. ATPase by 80% (I50 = 5.5 +/- 0.3 mM). Spermine inhibited enzyme decreasing its turnover rate and affinity for Ca2+. The ATPase affinity for Mg2+ increased in the presence of spermine. It was revealed, that the inhibitory effect of spermine is changed by the stimulatory effect under the increase of Ca2+ concentration (up to 2.6 microM), that correlates with the relaxing effect of this polyamine on the smooth muscle. |
Databáze: |
MEDLINE |
Externí odkaz: |
|