Acrylamide polymerization kinetics in gel electrophoresis capillaries. A Raman microprobe study.

Autor: Rapp TL; Department of Chemistry, University of Michigan, Ann Arbor 48109-1055., Kowalchyk WK, Davis KL, Todd EA, Liu KL, Morris MD
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 1992 Oct 15; Vol. 64 (20), pp. 2434-7.
DOI: 10.1021/ac00044a023
Abstrakt: The formation of 3.5% T, 3.3% C cross-linked polyacrylamide is monitored in 75-microns-i.d. electrophoresis capillaries by Raman microprobe spectroscopy. The disappearance of the acrylamide 1292-cm-1 band is followed with 60-s time resolution for 30 min, and 2-4 min resolution for up to 10 h. Polymerization is 98% complete in 1.5 h and greater than 99% complete after 2 h. In the 900-1700-cm-1 region no bands attributable to cross-linking are observable. Reaction in the capillary follows second-order kinetics. The reaction is faster in the bulk system because heat dissipation is not sufficient to maintain a constant temperature.
Databáze: MEDLINE